Figure 1

A region of an overlay of five ¹⁵N-HSQC spectra at increasing ligand concentrations. Each peak is represented by its contours. Red peaks correspond to the unbound protein; yellow to the protein at 1:8 saturation; green to 1:4; blue to 1:2; and magenta to the fully saturated protein. The maxima of the red peaks are labeled by crosshairs and residue numbers. The ligand is unlabeled, so its peaks are not present.