Figure 3
From: Characterization of an inducible promoter in different DNA copy number conditions
Characterization of the lacIQ constitutive promoter in RPUs. The ratio between the activities (S cell ) of lacIQ and J23101 promoters, measured via IQ RFP and 101 RFP devices respectively, was computed to obtain the lacIQ RPUs in the four copy number conditions investigated in this work. Consider that IQ RFP and 101 RFP have a different DNA scar between promoter and RBS. Unpublished data from our lab showed that the scar present in IQ RFP systematically overestimates promoters activity by 1.43-fold when compared to the scar in 101 RFP . Error bars represent the 95% confidence intervals of the mean value computed on 3 clones.