Induction curves as a function of the activated complex and correlation between K
and n. Induction curves for the HSL-inducible system as a function of the activated complex HSL-LuxR in different copy number contexts (Panel A) and correlation between the estimated dissociation constant of HSL-LuxR complex and luxI promoter (K
parameter) and copy number (Panel B). The activity of the luxI promoter, in terms of RPUs, was studied in each copy number condition as a function of its actual inducer, i.e. the HSL-LuxR activated complex (A), indirectly measured by an ad-hoc mathematical model described in the Methods section.