Table 1 Strains, plasmids and biological devices used in this work.
From: Characterization of an inducible promoter in different DNA copy number conditions
Strains | |||
|---|---|---|---|
Name | Genotype | Source | |
DB3.1 | F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20 ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1 | Invitrogen | |
BW23474 | F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510 | CGSC, Yale University, USA | |
TOP10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ 80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- | Invitrogen | |
MG1655 | F- λ-ilvG- rfb-50 rph-1 | CGSC Yale University, USA | |
MG-HSL RFP | MG1655, Φ80(HSL RFP ) | This study | |
MG-101 RFP | MG1655, Φ80(101 RFP ) | This study | |
MG-IQ RFP | MG1655, Φ80(IQ RFP ) | This study | |
Vector backbones used for RFP expression or genomic integration of the devices | |||
Name | Replication origin | BioBrickâ„¢vector code | Antibiotic resistance |
pHC | pUC19-derived pMB1 origin (high copy) | pSB1A2 | Ampicillin |
pMC | pMR101-derived p15A origin (medium copy) | pSB3K3 | Kanamycin |
pLC | pSC101 origin (low copy) | pSB4C5 | Chloramphenicol |
pΦ80 | R6K conditional origin, used for integration | BBa_K300000 | Chloramphenicol |
Genetic devices | |||
Name | Description | ||
HSL RFP | HSL-inducible mRFP1 expression system | ||
HSL GFP | HSL-inducible GFPmut3b expression system | ||
101 RFP | Standard reference constitutive promoter with mRFP1 expression device downstream | ||
101 GFP | Standard reference constitutive promoter with GFPmut3b expression device downstream | ||
IQ RFP | lacIQ constitutive promoter with mRFP1 expression device downstream | ||