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Table 1 Strains, plasmids and biological devices used in this work.

From: Characterization of an inducible promoter in different DNA copy number conditions

Name Genotype Source
DB3.1 F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20 ( r B - , m B - ) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1 Invitrogen
BW23474 F-, Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510 CGSC, Yale University, USA
TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ 80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- Invitrogen
MG1655 F- λ-ilvG- rfb-50 rph-1 CGSC Yale University, USA
MG-HSL RFP MG1655, Φ80(HSL RFP ) This study
MG-101 RFP MG1655, Φ80(101 RFP ) This study
MG-IQ RFP MG1655, Φ80(IQ RFP ) This study
Vector backbones used for RFP expression or genomic integration of the devices
Name Replication origin BioBrick™vector code Antibiotic resistance
pHC pUC19-derived pMB1 origin (high copy) pSB1A2 Ampicillin
pMC pMR101-derived p15A origin (medium copy) pSB3K3 Kanamycin
pLC pSC101 origin (low copy) pSB4C5 Chloramphenicol
pΦ80 R6K conditional origin, used for integration BBa_K300000 Chloramphenicol
Genetic devices
Name Description
HSL RFP HSL-inducible mRFP1 expression system
HSL GFP HSL-inducible GFPmut3b expression system
101 RFP Standard reference constitutive promoter with mRFP1 expression device downstream
101 GFP Standard reference constitutive promoter with GFPmut3b expression device downstream
IQ RFP lacIQ constitutive promoter with mRFP1 expression device downstream