In-silico and in-vivo analyses of EST databases unveil conserved miRNAs from Carthamus tinctorius and Cynara cardunculus

Table 1 List of primer sequences for RT-PCR analysis for cloning and sequencing microRNAs from Cynara cardunculus leaves

Related miRNA	Primer Name	Primer Sequence
ath-miR833-5p	mir833	cgccgc tgtttgttgtactcggtctagn
ath-miR157b	mir157	cgccgc ttgacagaagatagagnncac
ath-miR472	mir472	cgccgc ytttycctactccyccnatncc
ath-miR390b	mir390	cgccgc aagctcaggagggatagcnnc
ath-miR171b.c	mir171	cgccgc ttganccntnccaanatcacg
ath-miR171a	mir171a	cgccgc tgattgagccgcgccaatnnn
ath-miR832-5p	mir832	cgccgc tgctgggatcgggaatcnann
ath-miR169d	mir169	cgccgc tgagccaaggatgnnttgcnn
ath-miR397b	mir397	cgccgc tcatngagtgnancgttgatn
ath-miR164a	mir164	cgccgc tngagaagcagggnacntgca
	Poly(T) adapter	gcgagcacagaattaatacgactcactatagg(t)12vn §
	Poly(T) adapter reverse*	gcgagcacagaattaatacgact

§ degenerated bases are indicated by the following one-letter code: C+T = Y; A+C+G = V; A+C+G+T = N.
Motif cgccgc corresponds to stabilisation tail.
* Common reverse primers for all miRNAs

ISSN: 1471-2105