Table 2 Derivation of kinetic constants

IF1 + R > RBS1

10⁸ M^-1s^-1

[36]

RBS1 > IF1 + R

2.25 s^-1

[36]

IX > eR + IF1 + IF2 + IF3 + GDP + Pi + BS

0.18 s^-1

from frequency of new elongating ribosomes appearing on mRNA;

[37]

IX > RBS + fMetRNA

0.23 s^-1

from codon detection kinetics;

[38]

EFtu + EFts > tuts

10⁷ M^-1s^-1

[39]

tuts > EFtu + EFts

0.01 s^-1

[39]

EX > eRa + EFtuGDP + Pi

15 s^-1

from commonly accepted value of ribosome translocation rate (45 codons/s)

EX2 > EFg + GDP + Pi + eRp + tRNA

15 s^-1

from commonly accepted value of ribosome translocation rate (45 codons/s)

EX2 > EFg + GDP + Pi + tRNA + eRSTOP

0.05 s^-1

from length of GFP (~300 aminoacids) and ribosome translocation rate (45 codons/s)

aaSyn + ATP > AX

10⁸ M^-1s^-1

diffusion limit

AX2 > aatRNA + Syn

0.84 s^-1

from enzyme activities of the PURE system, as reported in [24]

AK + ATP > Y

10⁶ M^-1s^-1

from Adenylate Kinase Km for ATP, as reported in [40]

Y > AK + ATP

40 s^-1

from Adenylate Kinase Km for ATP, as reported in [40]

Y > AKP + ADP

300 s^-1

[41]