Comparison of PSI estimates. Comparison of PSI estimators of different methods for (a) high- (b)medium- and (c) low-cover junctions in a reference RT-PCR study. Each method's estimated distribution over PSI is shown in different color, and the target PSI value is shown as a yellow star on the x-axis. Methods which commit the most of their distribution mass near the star have the most accurate estimates. The text inside each plot identifies a cassette event and gives the raw number of reads mapping to the constitutive (Ne) and the average of the alternative junctions (Ni). This figure is best viewed in color.