Localization of IRA1 using heat tolerant replicates 1 and 2. The red and blue lines and shaded regions show the inferred allele frequencies in the two replicates using the DBN method, and the pluses plot the observed allele frequencies. The green line shows the LOD scores calculated using the DBN two-pool method. The gray box shows the position of IRA1, the reported and verified association in this region. However, an uncharacterized association upstream of IRA1 may be the cause of the extended range of low allele frequencies and the shifted estimate of the peak location.