Figure 3

Example output of Mojo Hand. a) Screen-shot of output from Mojo Hand for zebrafish gene flt3 Exon 1. Of all possible TALEN binding sites, this is Site 99. The record includes the binding sequences in proper orientation, the RVDs (tab delimited in normal output for easy transfer to a spreadsheet) and the restriction enzymes that could be used. Note that this example has only a single cutting site so the absolute position of the beginning of the restriction site is given but the column for relative position of the second cut is empty. b) Screen-shot of downloaded spreadsheet in .CSV format of the same example.