Personae were developed by the stakeholders and refined by user interviews. Two examples of the personae we created are (a) ‘Eunice’, and (b) ‘Debra’. Both represented bench scientists, with Eunice working in enzymology, and Debra in drug discovery. These personae, and three others shown in the Additional files 3 (‘Eric’ and and ‘Dean’) and 4 (‘Brenda’), reflected the expected desires and behaviours of potential users of the Enzyme Portal website. We described them in terms of name, role and motivations for using an enzyme resource.