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Table 4 AA changes in the ten best predicted prototypes for the broadly-neutralizing anti-influenza antibody CH65

From: MAPs: a database of modular antibody parts for predicting tertiary structures and designing affinity matured antibodies

  Position Antibody P1 P2 P3 P4 P5 P6 P7 P8 P9 P10
VH 1 E Q Q Q Q Q Q Q Q Q Q
36 D G G G G G G G G G G
38 H Y Y Y Y Y Y Y Y Y Y
39 I M M M M M M M M M M
40 N H H H H H H H H H H
57 H N N N N N N N N N N
64 D G G G G G G G G G G
83 A S S S S S S S S S S
91 V L L L L L L L L L L
92 N S S S S S S S S S S
93 G R R R R R R R R R R
95 K R R R R R R R R R R
107 G V G G G G G G G G G
108 G Q S I W G G G G G Y
109 L L I V I I I I I I S
110 E E A G Q A T T T T S
111 P R A A L A G G G G S
111A R R R T W A T T T T W
111B S S P T L T T T T T Y
111C V D D D H D D D D D D
112C D Y Y Y Y Y Y Y Y Y Y
V-LAMBDA 2 S Y Y Y Y Y Y Y Y Y Y
17 Q Q K K Q Q K K Q K K
27 D N N N N N N N N N N
36 R S S S S S S S S S S
42 N Y Y Y Y Y Y Y Y Y Y
54 V V I V V V I V V I V
55 C Y Y Y Y Y Y Y Y Y Y
56 Y D Y D D D Y D D Y D
116 V V V V W A W W A A A
117 I V V V V V V V V V V
123 K K K K K Q K K Q Q Q
126 V V V V V V V V A V V
  1. The AA changes of the top ten prototype (P) sequences (21 VH, 12 V-LAMBDA positions) are listed. In VH all positions outside of CDR3 have the same AA listed because the same V gene was selected. Within CDR3 different D gene selections give rise to different AA changes. In V-LAMBDA different V and J gene selections give rise to different AA changes throughout the entire domain. Sequence numbering is according to the IMGT® unique numbering [36-39].