Figure 2
From: FusionQ: a novel approach for gene fusion detection and quantification from paired-end RNA-Seq
Flowchart of FusionQ. FusionQ constructs the reference transcriptome directly from the RNA-Seq data, and detects the two types of fusion reads based on it. The proposed residual sequence extension method makes the short residual sequence longer, and therefore has a higher probability for unique mapping and increases the specificity of the detection results. The chimerical transcript quantification is based on the RSEM algorithm.