Figure 3

Cmap rank in CAGC genes. Panel A shows the gene rank distribution for all genes and all cmap instances (green curve) and the distribution of the median gene rank of each CAGC whose center is a known drug target (red curve). Note that the gene rank is defined as the highest rank among the probesets associated to the gene, which explains the non-uniform distribution of all genes. As an exemplar case, in panel B we show a box-plot comparing, for the cmap instance n. 436 (1e-05 M of Fasudil for 6 h in PC3 cells), the genes in the CAGC of ROCK1 (a known target of Fasudil) with all the other genes. The rank of the CAGC genes is significantly higher (Pvalue < 3.4e-05 Mann-Whitney U test), implying that the inhibition of the CAGC center by the drug leads to a general overexpression of the genes that compose its CAGC.