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Table 2 The rating system

From: Seq2Ref: a web server to facilitate functional interpretation

Feature

Criterion

Points

  

True

False

NA

Reciprocal BLAST

Query-to-hit-genome1 best hit

+1

0

+0.253

 

Hit-to-query-genome2 best hit

+1

0

+0.253

Multiple Sequence Comparison (MSC) Method4

Accept with identity cutoff 60%

+0.5

0

\

Accept with identity cutoff 50%

+0.5

0

\

Accept with identity cutoff 40%

+0.5

0

\

Pairwise comparison to the query4

Identity>60%

+0.5

0

\

Identity>50%

+0.5

0

\

Identity>40%

+0.5

0

\

others

Reference proteins

+1

0

\

  1. 1: use the input sequence to BLAST against the genome of the hit.
  2. 2: use the hit to BLAST against the genome of the input sequence.
  3. 3: the whole genome sequences of the protein are unavailable.
  4. 4: coverage in both the input sequence and the hits must larger than 80%.