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Table 2 The rating system

From: Seq2Ref: a web server to facilitate functional interpretation

Feature Criterion Points
   True False NA
Reciprocal BLAST Query-to-hit-genome1 best hit +1 0 +0.253
  Hit-to-query-genome2 best hit +1 0 +0.253
Multiple Sequence Comparison (MSC) Method4 Accept with identity cutoff 60% +0.5 0 \
Accept with identity cutoff 50% +0.5 0 \
Accept with identity cutoff 40% +0.5 0 \
Pairwise comparison to the query4 Identity>60% +0.5 0 \
Identity>50% +0.5 0 \
Identity>40% +0.5 0 \
others Reference proteins +1 0 \
  1. 1: use the input sequence to BLAST against the genome of the hit.
  2. 2: use the hit to BLAST against the genome of the input sequence.
  3. 3: the whole genome sequences of the protein are unavailable.
  4. 4: coverage in both the input sequence and the hits must larger than 80%.