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Figure 1 | BMC Bioinformatics

Figure 1

From: Dynamical modelling of phenotypes in a genome-wide RNAi live-cell imaging assay

Figure 1

Examples from the Mitocheck screen data. (a) Images of HeLa cells imaged 18 h and 42 h after seeding on a control spot containing siKIF11, an siRNA targeting the kinesin gene KIF11. Interphase cells are outlined in white. Inhibition of KIF11 induced prometaphase arrest and led to an accumulation of mitotic cells (red). Of the arrested cells many subsequently became polynucleated (green) and eventually died (blue). (b) Time courses of cell counts in four spots. The negative control siRNA siScrambled led to normal cell growth in two replicated spots (top). siKIF11 led to an early accumulation of mitotic cells, while at later times many of the arrested cells went into cell death (bottom-left). siCOPB1, which targets an essential Golgi-binding protein, caused cell death, but no mitotic phenotypes (bottom-right). Y-axes scales were scaled to accommodate the different dynamic ranges of the data.

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