Figure 3
From: Dynamical modelling of phenotypes in a genome-wide RNAi live-cell imaging assay
Model fits provided meaningful biological parameters that helped annotate gene function. (a) Box plots of cell death penetrance αD estimated in control and sample wells. Lethal phenotypes induced by siKIF11 and siCOPB1 had significantly higher cell death penetrance than the negative siScrambled control spots. (b) Cell count time courses of two spots containing an siRNA (MCO_0026491) targeting the not well-characterised gene C3orf26. Induction of cell death started at τD = 30.8 h and 33.8 h. (c) Box plot of mitosis duration estimated in control and sample wells. Prometaphase arrest caused by siKIF11 led to a significantly higher mitosis duration than cells in negative control spots. (d) Cell count time courses of two spots containing an siRNA (MCO_0020444) targeting the poorly characterised gene CCDC9. The overall high proportion of mitotic cells, compared to the negative controls illustrated in Figure 1b, is indicative of a longer mitosis duration, estimated at 5.7 h.