Kinetic Flux Balance Analysis. Comparisons between FBA and KFBA predictions for independent test conditions using a central (A,B) or genome-scale constraint-based metabolic model (C,D). (A) Bar height indicates mean residual for each method in the five test conditions using a central metabolic model. Residual was calculated as the squared difference between measurement and FBA or KFBA prediction. (B) Bar height indicates the biomass yield from experiments or predictions from FBA or KFBA using a central metabolic model. (C) Residuals when using the iJO1366 model in KFBA for different numbers of kinetic flux bound violations (n*). When all flux bounds are ignored the KFBA solution is equivalent to the FBA solution. (D) Biomass yields when using the iJO1366 model. KFBA yield predictions (green) correspond to the yield that results when the minimum number of kinetic flux bound violations are allowed.