Figure 4From: Blind spots of quantitative RNA-seq: the limits for assessing abundance, differential expression, and isoform switchingIsoform length dependence of the alignment rate in the presence of variable transcript start sites and poly-A tails. If the transcripts are simulated with poly-A tails and variable transcript start sites, the mapping rate of the short isoforms is low. For the visualization at the log-scale we offset the counts by 1 and a small jitter.Back to article page