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Figure 8 | BMC Bioinformatics

Figure 8

From: Blind spots of quantitative RNA-seq: the limits for assessing abundance, differential expression, and isoform switching

Figure 8

Receiver-operator-characteristics for the detection if isoform switching. The performance strongly depends on the alignments and the testing algorithm. In our comparison DEXSeq performs best. DiffSplice only performs well with tophat alignments but not with STAR alignments. The curves do not cover the full range of specificities because algorithms like DEXSeq and cuffdiff only run a test for a given gene locus if the locus satisfies additional constraints on read coverage. If a test was not run, it was considered as no splicing detected.

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BMC Bioinformatics

ISSN: 1471-2105

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