Simulated time course of species in the crosstalk model of IL-6 after pre-treatment with IFN-gamma or IFN-gamma after pre-treatment with IL-6. (A) Pre-treatment with IFN-gamma significantly decreased the activation of STAT3 induced by IL-6. (B) Without SOCS3, the inhibitory effect of IFN-gamma on STAT3* induction by IL-6 was completely eliminated. (C) Temporal pre-treatment with IFN-gamma partly inhibited IL-6 signalling. (D) Pre-treatment with IL-6 reduced the amount of STAT1* only slightly and did not inhibit the response of IFN-gamma. (E) Changing the length of pre-treatment with IL-6 did not affect the signal response of IFN-gamma. (F) Without the formation of STAT1/3 heterodimers, IL-6 had a higher capacity to inhibit IFN-gamma signal transduction. (G) Without the formation of STAT1/3 heterodimers, IFN-gamma had a higher capacity to inhibit IL-6 signal transduction. (H) Abolishing (STAT1N)*2 induction of SOCS3 inhibited the IFN-gamma effects on IL-6.