Figure 2

Data simulation and smoothing performance. a.) Heat maps of 100,000 probes derived from DNA methylation profiling of 5 control hippocampal samples from OVX C57BL/6 J mice and a simulated data matrix where probe mean and probe variance were modeled on the empirical dataset. An example of a simulated DMR inserted data matrix is depicted. b.) A plot of an example DMR 5 probes long exhibiting an average log₂ fold change of 1. Mean group-wise differences are plotted in black (y-axis, top) as a function of the relative probe position (x-axis, top). The smoothed t-statistics (left y-axis, middle, red) generated by the CHARM algorithm and the relative meta-analysis weights generated by BioTile (right y-axis, middle, blue), are plotted. A heat map of the location is depicted (bottom). Vertical green lines denote the location of the inserted DMR.