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Table 2 Fold-changes between thrombin-treated samples and control samples (SRA: SRP008482).

From: Assessing the impact of human genome annotation choice on RNA-seq expression estimates

 

RNA-seq data with TPM Expression Estimates (OSA Pipeline)

Gene

RT-qPCR

AceView

H-InvDB

Ensembl

Vega

UCSC

RefSeq

TRAF1

2.862

3.029

3.034

3.025

2.998

2.934

2.922

FANCD2

-1.050

-0.782

-0.687

-0.888

-0.856

-0.840

-0.840

CELF1

-0.202

-0.138

-0.202

-0.098

-0.098

-0.239

-0.275

Average Absolute Deviation from RT-qPCR

0.166

0.178

0.143

0.145

0.106

0.114

 

RNA-seq data with FPKM Expression Estimates (TopHat/Cufflinks Pipeline)

Gene

RT-qPCR

AceView

H-InvDB

Ensembl

Vega

UCSC

RefSeq

TRAF1

2.862

3.874

3.845

3.797

3.719

3.677

3.674

FANCD2

-1.050

0.057

0.057

-0.345

-0.322

-0.202

-0.151

CELF1

-0.202

0.642

0.516

0.595

0.585

0.390

0.356

Average Absolute Deviation from RT-qPCR

0.987

0.936

0.812

0.791

0.751

0.756