Table 1 Microarray vs. RNA-seq

Principle

Hybridization

Cloning & sequencing

Required amount of RNA

High

Low

Resolution

Several to 100 bp

Single base

Distinguish Allelic expression?

Limited

Yes

Distinguish splice forms?

Limited

Yes

Discover new genes?

No

Yes

Strandedness?

No

Yes

Dynamic range

Few hundred-fold

> 8000-fold

Reproducibility

Yes

Yes

Cost

Medium

High (due to computation)