Figure 1

Schematic diagram of pre-miRNA, hsa-mir-200c, predicted by quikfold software and patterns of Dicer cleavage site at 5p arm. (A) miR* derived from 5p arm and miR derived from 3p arm of hairpin, bases are represented in capital letter. CD-5p and CD-3p are cleavage sites of Dicer at 5' and 3' arm respectively. (B) Sequence of CP-5p cleavage pattern of 14 nucleotides having cleavage site CD-5p at center. Following each cleavage pattern, features of mononucleotide and binary used as input feature for SVM are given. (C) Structure of CP-5p cleavage pattern of 14 nucleotides having cleavage site CD-5p at center and its partially complementary strand. Base pairs are indicated with arrows. Zero (0) indicates that no base pairing occurs between complementary strands. The pattern of 14+14 is used to generate binary pattern. Mononucleotide having 4, sequence binary pattern having 56, and structure binary pattern having 112 dimensional vector. +1 is the class for cleavage pattern. Binary pattern is represented only for highlighted nucleotides.