Water distribution patterns in HsTIM wild type and E104D mutant dimer interfaces. The water molecules in this interface are shown in blue spheres, and the non-interface region is in green. The interface are divided into four regions: regions A, B, C and D, and they are outlined by color magenta, orange, red and yellow, respectively. Subfigures (A) and (C) show one side of the wild type and mutant interface (in surface), respectively, and (B) and (D) show side views of the wild type and mutant interface, respectively. The residue GLU104 (in (A) and (B)), and ASP104 (in (C) and (D)), which are in region A, are highlighted in black.