HCS-Neurons: identifying phenotypic changes in multi-neuron images upon drug treatments of high-content screening

BMC Bioinformatics

Table 5 Anova analysis of the effect of nocodazole concentration on the neuron feature descriptor.

Feature Name	ANOVA R ² (%)	Nocodazole concentration (ng/mL)						Scale (pixels)
		0	10	50	100	200	1000
somaCount	65.9	224^A	249^B	255^B	251^B	221^A	137	x1
somaArea	62.3	568^A	716^B	867^C	970^D	949^CD	591^A	x10²
neurite Length	94.4	808	598	385	251	156	89	x10²
neuriteArea	95.3	203	164	119	80	49	27	x10³
attachmentPoint#	69.5	126^AB	138^BC	140^C	136^BC	115^A	64	x10
endingPoint#	82.5	440	345	270^A	255^A	216	147	x10
branchPoint#	91.0	493	336	209	152	109	75	x10²
Avg_somaArea	76.8	250	287	340	388	431^A	432^A	x1
Avg_neurite Length	88.0	372	244	151	100	71^A	65^A	x1
Avg_neurite Area	91.3	924	666	470	317	222^A	198^A	x1
Avg_attachment Point#	58.4	562^A	554^AB	546^AB	542^B	520	468	x10^-2
Avg_ending Point#	68.6	203	141	106^A	102^A	98^A	108^A	x10^-1
Avg_branch Point#	85.1	227	137	82	60^A	50^A	55^A	x10^-1

Values with the same superscript letter are not different at the 5% experiment-wise level. Values with no superscript are different from all other values for that feature. The R² ANOVA value is the fraction of variation that occurs between groups versus the total variation for that feature. For 216 data measurements values of R² over 11.2% show a significant difference between group means.

ISSN: 1471-2105