Empirical and Gaussian probability density functions of the height ratio. The statistical analysis was performed to determine the threshold value of the height ratio for eliminating residual objects. It can be observed that the empirical probability density function can be approximated by Gaussian model. From the Gaussian model, the objects are classified as residual objects when Hi
< 0.7507. When Hi
≥ 0.7507, the objects are classified as mixing between skewed objects and touching/overlapping chromosomes.