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Table 3 Local structural alignment of the residues in the GH16 consensus signature positions for the known representative GH16, endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase, with the SG protein YP_001304206.1.

From: Protein function annotation with Structurally Aligned Local Sites of Activity (SALSAs)

Spatial Positions→

1

2

3

4

5

6

7

8

9

10

11

12

13

14

2ayh (GH16)

F92

Y94

W103

d104

E105

D107

E109

L111

Q119

N121

Y123

Y147

W158

W184

3h3l (SG)

F125

-

Y138

-

s140

E142

q144

L146

A166

Y168

-

t187

H198

H256

  1. Previously reported active site residues are shown in boldface. POOL-predicted residues (top 8%) are shown in uppercase; residues not predicted are shown in lowercase. The poor match suggests different functions.