Novel AS Events | Carraro et al. | Carraro et al. (*) | NGS-Trex (N > 1) | NGS-Trex (N > 2) |
|---|
Total | 2865 | 1757 (**) | 1641 | 683 |
Canonical | na | na | 1245 | 539 |
RT-PCR validation | 18 | 36 | 36 |
qRT-PCR | 8 | 16 | 16 |
- As described more in detail in the text, NGS-Trex treats splicing events from the "intron point of view" while in Carraro work splicing are classified from the exon perspective. This implies that one cassette-exon event is identified by NGS-Trex as two splicing sites. To compare data we corrected data to take into account this difference. Column marked with (*) shows the corrected values. (**) 1757 events derive from 530 AS donor/acceptor site usage + 487 exon skipping + 562 exon inclusion + 89×2 exon inclusion (each exon inclusion needs 2 introns). See also Figure 4 in Carraro paper for details.
