Screen capture of an rpo B query: DNA alignment and detected mutations. The figure shows an example of the detection of a mutation. The whole nucleotide alignment can be observed by horizontally scrolling the alignment window. In the case of the rpoB gene mutation, the nucleotide positions in M. tuberculosis and E. coli are indicated. The protein scheme compares the wild-type and query sequences for a given position. This scheme describes the changes along the protein sequence and emphasizes the effect of the mutation on the protein chain.