A typical example of interactive and cross-species GSA in gsGator. A. Preparation of input gene set using set operation, orthology mapping, and network expansion tools. Two human gene sets (H1, H2) are combined to generate H1∩H2 by intersection operation. An orthologous set of M1 is generated by orthology mapping to mouse genes from H1∩H2. The M1 set is expanded via a molecular network, resulting in M2. B. GSA is performed between predefined gene sets in the public category and M1, M2 in the private category. The GSA result is shown as a table or a network among gene sets, where significantly associated gene sets are connected to each other.