Table 1 Lists the experimentally validated novel microRNAs and their putative targets in different signaling pathways at EL −25 ΔG kcal/mol

a.

Novel miRNA

Targets(Jak-Stat)

hsa-miR-193b-5p

SOCS-2, AKAP13, AKT2, BCL2L1, CLCF1, IL10RA, IL10RB, JAK3, LIF, PIAS4,PIK3R2, PIK3R5, PRLR, PTPN11, SOCS7, STAT5B, TSLP, TYK2

hsa-miR-296-3p

AKT2, BCL2L1, CSF3, GRB2, IFNGR2, IL10RA, IL11, IL11RA, IL9R, PIK3CB, PIK3R2, PIK3R5,PRKAR2A, PTPN6, STAT5A, STAT6, TSLP, TYK2

hsa-miR-296-5p

CREBBP, GRB2, PIAS2, PTPN11, SOCS3, STAT6

hsa-miR-339-3p

IL20RA, SOCS2, STAT5A, CLCF1, GH2, GHR, IL2RB, IL4R, IL6R, LIF

hsa-miR-491-5p

CBLB, CISH, CSF3R, PIK3R2, PTPN6, SOCS7, SPRY4, TYK2, AKT1, IKBKG, PIK3R5, SERF2, AKAP13, CSF3, IFNAR2, IL15RA, IL2RB, IRF9, LEP, PIK3R5, PRLR, SOCS2, SPRY3, STAT6, STS

hsa-miR-623

AKAP13, AKT2, BCAS1, CSF3, CSF3R, GRB2, IFNAR2, IL10RA, IL11RA, IL28RA, IL4R, IL6ST, PIK3R2, PIK3R5, SASH1, SOCS3, SPRY4, STAT5A, STAT6, TYK2

hsa-miR-639

CCND1, IL2RB, PIAS4, PRLR, AKAP13, BCL2L1, CBL, CISH, CNTFR, CSF2RB, CSF3, GRB2

hsa-miR-770-5p

IL4R, PIK3R3, AKAP13, AKT2, CBL, CNTFR, CSF3, IL11RA, IL6ST, IL7R, PIK3R5, PTPN11, SOCS2, STAT5A, STAT5B, STAT6, STS, TYK2

hsa-miR-941

CSF2RA, CTF1, IFNAR1, IFNAR2, IL10RA, IL10RB, IL11RA, IL28RA, LIF, OSM, PIK3CA, PIK3R2, PRKAR2A, PRLR, STAT5A, SOCS3, SPRED2

hsa-miR-15a-3p

BCAS1, FZD4, PIK3R1, AKAP13, BCAS1, CISH, CSF2, CSF3R, GRB2, IL13, IL13RA1, STAT5A, TYK2

hsa-miR-141-5p

AKT2, BCL2, PRKAR1B, TRAF2, XIAP, CISH, IL22RA2, PIK3R3, PIM1, STAT2, STAT6

hsa-miR-148b-5p

IFNAR2, JAK1, DFFA, IFNAR2, JAK1

hsa-miR-17-3p

EXOG, IRAK3, TRAF2, CBL, STAT1 IL15RA, IL19, STAT6

hsa-miR-188-3p

AKT1, OSMR, SPRY2, SPRY4, CCND1,IL10RA, CBLB, CISH, CNTFR, CSF2RB, IL13, IL13RA1, IL6, IL9R, PTPN6, SPRY3, STAT3, TYK2

hsa-miR-218-2-3p

AKAP13, AKT2, PIM1, STAT5A, TYK2, CSF2RB, CSF3R

hsa-miR-219-1-3p

EXOG, AKAP13, AKAP13, IL7R, PIM1, SPRY3, TSLP, IL11, IL13RA1, MATR3, IL19

hsa-miR-24-1-5p

PIK3R1, IL19, CSF2RB, DFFB, PIK3R1, NRG1

hsa-miR-329

SOCS2, IL10RA, IL4R, GRB2, IFNAR2, IL11RA , JAK3

hsa-miR-337-5p

AKT2, CBL, IL10RB, TNFRSF10B, AKAP13, AKT1, CSF2RB, IL10RA, IL13RA1, PIK3R5, STAT6, CTF1, IL28RA, JAK3, STAT5A, STAT5B, STS, TYK2 , MVP

hsa-miR-506-3p

PIAS2, STAT5A, CCND2

hsa-miR-519e-3p

AKT2, AKT1, STAT3, STAT5A, IKBKG, NF2

hsa-miR-521

AKT1, PIK3R1, STAT5A, CBL, CCND2

hsa-miR-550a-3p

AKT1, CSF3, JAK3, STAT3, CASP7, DFFA, DFFB, EXOG, IKBKB, IRAK4, PPP3R1, PRKAR2A, IL10RA, IL28RA, IL2RB, PRKAR2A, IL6R

hsa-miR-617

IL11, IL19, IL4R, LIF, PRLR, SOCS7, IL15, IL6R, AKT2, IL21R, STAM2, STAT3, IKBKG, XIAP

hsa-miR-644a

AKAP13

hsa-miR-934

CSF3, IL11RA, LIF, PTPN11, SOCS2, PIK3R2, AKT3, OSM, TYK2, APAF1, CASP7, IL1R1, SERF2, TRAF2

hsa-miR-147b

XIAP, PIK3CG, STAT5B, CCND1, CCND3, CISH, CLIC6, IL19, JAK1, SPRED1, TYK2

hsa-miR-33a-3p

IL15, EXOG

hsa-miR-146b-5p

AKT1

hsa-miR-19a-5p

CBL

hsa-miR-26a-1-3p

AKT2, TYK2, PIAS3

hsa-miR-493-5p

AKAP13, KIT

hsa-miR-562

IKBKB, ADAM17, NMI, TGFB1, TNFSF13B, PTK2, AKT2, CSF3, KIT

b.

Novel miRNA

Targets (MAPK)

Targets(NOTCH)

Targets (VEGF)

Targets (Wnt)

hsa-miR-296-3p

F2RL1, NFATC2, EPHB4

EPHB4

F2RL1, NFATC2

NFATC2, VANGL1

hsa-miR-296-5p

APC, CSPG4

-

AQP1

ZEB2, APC

hsa-miR-339-3p

WEE1, FLNA

-

-

CACYBP

hsa-miR-491-5p

PDGFRA, XIAP, KAT6B

-

LRP6, PDGFRA

XIAP, LRP6

hsa-miR-770-5p

-

MAGEA1

RBM39

-

hsa-miR-941

-

-

STAT5A

-

hsa-miR-15a-3p

ARF1, WNT5A

-

RBL2

SETD8, WNT5A, VIM

hsa-miR-141-5p

FLOT1

-

IL17F

SULF1

has-miR-148b-5p

LRP1

-

LRP1, KCNH1, DNMT1

LRP1, PLCB2

has-miR-17-3p

CXADR, DUSP22, PLK1

GDPD5

PLK1

-

has-miR-188-3p

YWHAZ

MUC16, GLI1

GLI1, PIK3R2, PIK3R2

GLI1

has-miR-218-2-3p

FGF4, TNC

-

TNC

CSNK1E, PLCB2

has-miR-219-1-3p

NOS2

-

NOS2

CHST11

has-miR-24-1-5p

NRG1

-

NRG1

-

has-miR-329

CDH13

JAK3

JAK3

-

has-miR-337-5p

RARA, MVP, DUSP22, VEGFA, ABCA1, GRIN1, MAPK12, F10, BCL6, IGFBP5

CDKN1A, NCSTN, VEGFA, WNT3A

VEGFA, MAZ, LIMK1, MAPK12, F10, COL1A1, PIK3R1, IL15, IGFBP5

AXIN2, MBD2, CDH11, WNT3A, BCL6

has-miR-506-3p

-

-

-

CCND2

has-miR-519e-3p

NFKB2, RARA, NF2, TFF2, ARRB2

ARRB2

-

ARRB2, NF2

has-miR-521

-

-

NFATC1

NFATC1

has-miR-550a-3p

DYNLL1, IL6R

LFNG

IL6R, IL15

ING4, SFRP5

has-miR-617

OCLN, LRP1, NDFIP1, TIMP1

NCOR1

OCLN, MMP2, LRP1, TIMP1, ARF6

LRP1, TIMP1

has-miR-644a

-

-

-

CUX1

has-miR-934

KCNMA1

GLI1

GLI1, APEX1

GL1

has-miR-33a-3p

FLT4

FLT4

FLT4

AXIN1

has-miR-146b-5p

NFATC2, XIAP

-

NFATC2

XIAP, NFATC2

has-miR-26a-1-3p

AGER, ERBB, ACAT1, CXCR2, PRMT1, SMAD3, APOBEC3G, RRAS, PLAUR, LRP1, PGR, ERBB3, MAPK12, TRAF4,

NOS3, NOTCH4, PLAUR, FURIN

AGER, NOS3, NOTCH4, PARG, PLAUR, ERBB3, LRP1, CD47, MAPK12, FURIN, SMAD3

LRP1, ERBB3, MCC, SCN5A, PRMT1, ZNF703, SMAD3

has-miR-493-5p

PODXL, GRIN1, KIT, TGFB3

-

MUC1, KIT

FERMT2, IGF2BP1, PLCB2

has-miR-562

CDH5, PTK2, FLNA, SP1, CSF3, TGFB1, NFATC2, CD36, ERCC1, ADAM17, ARRB1, NGFR, HSPB1, MAP3K11, PTK2, TNFSF13B, APOB, KIT, CSF3, AKT2, TRAF2, ELK1, CD274, CAMK2B

ADAM17, ARRB1, TGFB1

ADAM17, APOB, HSPB1, PTK2, CSF3, AKT2, CDH5, TGFB1, NFATC2, PPARA, VASH1, SP1, KIT

CHST11, TGFB1, NMI, FXYD5, NFATC2, CAMK2B