Performances of individual and combined callers. Model fitting was done using a random 50% of the point mutations detected from the selected 20 patients, and evaluation was done based on the remaining half. In the main panel, the true positive and the false positive rates of various callers are shown: (1) three individual callers (red filled triangles): Caller A, Caller B, and Caller C, (2) the caller that cumulatively adds mutation sets based on the combination call status in the order of the validation rate (connected blue dots), (3) the combined caller built by fitting a logistic model (for details, see text) (green line). The area near the point showing Caller C’s performance is enlarged and shown as a small sub-panel on the lower right part of the main figure. This panel further indicates the performance of the callers that take unions or intersections of calls from three callers (brown diamonds): all callers (ABC), intersections of two callers (AB, AC, or BC), called by more than two callers (‘2orMore’).