Figure 3

Method implemented to correct GC content biases. Variations in size-corrected mean read numbers according to GC content. The polynomial equations are indicated above (A: Cattle sample 1479, and D: Drosophila sample SRR384925). Application of the previous equation (Eq.3) to differences between 50% GC content and each GC content value, giving the equation indicated above (Eq.4) (B: Cattle sample 1479, and E: Drosophila sample SRR384925). Effect of GC content bias correction on the whole dataset. Clearly, no remaining dependence can be observed: the p-value to third order polynomial equation is 1.00 (C: Cattle sample 1479, and F: Drosophila sample SRR384925).