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Table 2 Correction of the impact of total read numbers

From: An integrative method to normalize RNA-Seq data

 

N

Samples

log2(FPKM)

SGTR - Size

SGTR - Size and GC content

Full SGTR

All genes

159

1475

7,96E-39

1.71E-39

4.80E-39

1.08E-38

155

1455

2,95E-39

5.86E-39

3.40E-39

2.66E-39

162

1479

8,24E-44

1.37E-43

2.02E-49

1.21E-49

152

1345

1,57E-42

1.86E-42

6.11E-44

5.64E-44

 

162

1476

1,73E-41

6.74E-41

1.51E-44

2.28E-44

  1. N corresponds to the number of analyzed genes. The five samples (1475, 1455, 1479, 1345, and 1476) refer respectively to samples with a total read number around 10.106, 13.106, 20.106, 24.106, and 30.106 reads. Abbreviations: SGTR size: correction for transcript size; SGTR Size and GC content: correction for transcript size and GC content; and Full SGTR: correction for transcripts size, total read number, and GC content. Only the p-values of Pearson correlation with qRT-PCR quantifications are indicated.
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BMC Bioinformatics

ISSN: 1471-2105

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