Figure 3

ABFGP-based curation of the MFS transporter-encoding gene Cf189922 of Cladosporium fulvum. A. Selected tracks of the GFF results obtained by applying ABFGP on the Cf189922 gene locus using 17 fungal informant genes. The annotated (blue) and the ABFGP-predicted gene model (green and grey) are shown on top. The grey part of the ABFGP prediction indicates an intron-exon boundary with status ‘doubtful’. Below are indicated the introns (orange) and exons (red) that were revised; the red box highlights the site of the second revision. The intron evidence track lists intron-exon boundaries obtained from informants; the colours used in the informant gene similarity track represent a measure for pairwise amino acid similarity. The alignment similarity track represents a summed representation of the inferred multiple sequence alignment of all informants. B. Multiple protein sequence alignment of currently annotated gene models of Cf189922 and its informants. Sequence is restricted to the red box shown in panel A. C. Multiple protein sequence alignment of the ABFGP-revised gene model of Cf189922 and its informants. Sequence is restricted to the red box shown in panel A. The proposed revision is highlighted in the black box.