Table 1 Datasets tested using the GESS online tool and results
Study | RNAi (species) | Phenotype | No. active siRNA | Statistically significant OT gene |
|---|---|---|---|---|
A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability [16] | shRNA (hs) | Ionizing radiation sensitivity | 850 | ZNF480 SH3BP2 |
Ionizing radiation sensitivity (validated) | 114 | None | ||
Ionizing radiation resistance | 1080 | None | ||
An intermittent live cell imaging screen for siRNA enhancers and suppressors of a kinesin-5 inhibitor [17] | siRNA (hs) | Genes involved in spindle assembly checkpoint (Dharmacon library) | 308 | MAD2L1 |
Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2 [18] | siRNA (hs) | Genes involved in TGF-β signaling (Sloan Kettering Inst. In house library) | 409 | TGFBR2 |
Genome-wide siRNA screen identifies SMCX, EP400 and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression [19] | siRNA (hs) | Genes that contribute to the repression of the HPV LCR. (Dharmacon library) | 511 | None |
A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response [12] | siRNA (hs) | Regulator of homologous recombination (Dharmacon library) | 510 | Rad51 |
Regulator of homologous recombination (Ambion library) | 187 | None | ||
Kinase requirements in human cells: I. Comparing kinase requirements across various cell types [20] | TRC shRNA (hs) | Essential kinases in HeLa and 293 T cell lines. | 110 | None |
A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Array Viral High-Content Screen [21] | TRC shRNA (hs) | Genes required for mitotic progression and proliferation for HT29 cell line | 161 | None |
Highly parallel identification of essential genes in cancer cells [22] | TRC shRNA (hs) | Essential genes for 12 cancer cell lines | 182 | None |
Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer [23] | TRC shRNA (hs) | Essential genes specifically for Ovarian cell lines | ~1500 (top ~3%) | None |
Essential genes specifically for Colon cell lines | ~1500 (top ~3%) | None | ||
Essential genes specifically for Pancreas cell lines | ~1500 (top ~3%) | None | ||
Essential genes specifically for Esophageal Squamous cell lines | ~1500 (top ~3%) | TACO1 | ||
Depleting gene activities in early Drosophila embryos with the "maternal-Gal4-shRNA" system [24] | shRNA (dm) | Abnormal embryonic phenotypes | 79 | None |
A regulatory network of Drosophila germline stem cell self-renewal [25] | shRNA (dm) | Abnormal embryonic phenotypes | 329 | None |