The effect of PacBio RS long read coverage on genome closure. Results are displayed for SSPACE-LongRead based on the CLCbio draft assembly for 5 organisms. For all samples the addition of PacBio reads has a positive effect and leads to a significant contig reduction. In general a 50× coverage is sufficient to scaffold over most gaps, though ideally a 110-160× coverage is required to guarantee an optimal performance of our software. Arguably a higher coverage (>160×) leads to more fragmented genomes, which is likely due to the increased complexity of the assembly graph.