Bifurcation judgment and false negative resolution. a. A schematic overview of the semi-local neighborhood used to measure tentative bifurcations and choose their resolution. Sub-regions of the neighborhood that are particularly relevant to specific scenarios are colored accordingly. b. Resolution of a typical FN showing how the matching of the ends of a potential FN is performed. The top panel shows the links between detections of two cells, defining a semi-local neighborhood with a bifurcation in green and a NN claim that does not correspond to a link dotted. The lower panel illustrates how matching is evaluated at the putatively correctly segmented endpoints, ignoring links and detection events during the proposed FN (which if there is a FN are unreliable). Each possible match is represented by a different colored pair of dotted lines. c. Illustration of two dimensions of the high dimensional measurement vector used to distinguish different bifurcation causes. It illustrates the relatively consistent appearance of many bifurcations with a particular origin, but also that appearances vary widely in a minority of challenging cases.