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Table 6 Results for Fragilariopsis data for different combinations of segmentations methods and contour optimization

From: SHERPA: an image segmentation and outline feature extraction tool for diatoms and other objects

Otsu’s thresholding Histogram equalization RATS (σ = 3) RATS (σ = 1-11) Adaptive thresholding Canny edge detector Contour optimization Ranking 0 total Ranking 1 total Ranking 2 total2) Total ranking 0 to 2
       248 168 28 444
      248 223 99 570
        224 161 23 408
       224 230 73 527
        258 193 31 482
       258 287 97 642
        340 167 37 544
       340 271 97 708
        217 169 43 429
       217 264 126 607
        217 122 11 350
      217 141 19 377
     385 170 38 593
   385 249 91 725
    403 164 44 611
  403 248 95 746
    421 155 52 628
  421 243 97 761
  1. 2)Whilst results of ranking 0 and 1 contain nearly only correctly segmented valves of Fragilariopsis and a few of other species, ranking 2 also contains few results of girdle bands incorrectly identified as valves.
  2. The more methods are combined, the higher is the yield.