Identification of an uncharacterized conserved protein domain in AtVAD1 homologs. (A) Amino acid conservation score along an alignment of AtVAD1 13 homologs. The histogram shows conservation score for each position of the alignment; the red line shows 10-AA moving average. (B) Top: AtVAD1 protein schematic diagram with known domains (GRAM, glucosyltransferases, Rab-like GTPase activators, myotubularins; VASt, VAD1 analog of START; TM, transmembrane helix; and CC, coiled-coil). Residue number corresponds to amino acid position of AtVAD1 domains. Bottom: Genomic organization of AtVAD1 gene showing intron/exon structure. AA, amino acids.