Amino acid conservation in the VASt domain and domain architecture of VASt-containing proteins. (A) HMM logo of the 193 AA VASt domain. Relative entropy is measured in bits. High entropy values indicate a high degree of certainty that the corresponding amino acid is present in orthologs domain. AA are colored to represent structural or functional similarity. The width of the amino acid positions indicates the probability of insertions (wider) and deletions (narrower). (B). Domain architecture of VASt-containing proteins. Schematic representation of the proteins retrieved after 3 iterations using jackhmmer tool.