Phylogeny and evolutionary history of the VASt domain. (A) Maximum likelihood phylogenetic tree of 85 VASt protein domains from 17 fully-sequenced species representative of major Eukaryotic lineages. Multiple copies of VASt found in a single protein are linked with central connectors. Protein Domain architecture and taxonomy are shown along branches. Nine phylogenetic groups (G1 to G9) are highlighted. Proteins cited in the main text are labeled along branches. (B) Proposed scenario for the evolution of VASt-containing proteins. Domain symbols are as in A. LCEA, last common Eukaryote ancestor.