Figure 4

Differentiating members of the subgroup TVV1 using in silico melting curves. A region with variable RNA secondary structure (A) and a region with conserved RNA structure (B) were obtained from the alignment of ORF2 of all members of the group using the software RNAz. The curves were generated by RNAheat and MELTSIM to conserved regions (C) and variable regions (D). Each denaturation curve is marked with a different color: Dark blue lines to TVV1-C344; orange lines to TVV1-OC3; yellow lines to TVV1-OC4; green lines to TVV1-OC5; dark red lines to TVV1-UR1; light blue lines to TVV1-UH9 and dark green lines to TVV1_I.