Description of the algorithm. (A) Original image. (B) Image after edge detection. (C) Thresholding. (D) Skeletonization. (E) Cell selection from the previous frame thinned to the skeleton pixels (white line). (F) Pixels of the adjusted skeleton area for the skeleton in E. (G) Area probed for elongation (grey semicircles) and pole elongation pixels (green). (H) New cell area is reconstructed by smearing the disk of configured radius along the pixels from F and G yielding white and green area, respectively.