Figure 5

Visualisation and analysis of gene expression. (A) Lineage tree from the presented time-lapse movie. Color scale represents background-corrected mean green fluorescence in arbitrary units (AU). Cell area (μm²) is displayed as proportional to the branch width. Time is indicated on the vertical axis. Switch to the acetate-based carbon source of the growth medium (dashed horizontal line) induces strong expression of GFP, representing induction of the icl gene. (B) The average green-to-red fluorescence ratio calculated from all cells from three time-lapse movies. Throughout the course of the experiment there were 299 cells in total. Error bars represent standard deviation. Red vertical line indicates the time of switch of the carbon source in growth media from glucose to acetate.