A case study on the miR-34a overexpressed HCT116 cells. (A) Fold change of all genes and miR-34a targets. The p-value is estimated by K-S test. (B) Fold change and NPES score of miR-34a targets. Blue dot represents the absolute value of gene expression fold change, which is normalized by the maximum of all genes; Green dot represents the NPES computed by using single target as the RWR seed in the step 1 of forward searching, and all the miRNA targets are sorted according to this score; Red dot stands for the NPES generated by using multiple targets as the RWR seed, the peak value is the optimal score and those targets appearing at and before this point are the leading-edge targets. (C) Neighbor sub-network of the miR-34a target CUX1. Gene name and expression fold change are labeled in each node.