Alignment variations with and without TMAdv in the target library. A) Without the TMAdv present in the target library, Clinical PathoScope assigned reads to several adenovirus genomes. The identified genomes are displayed according to the proportion of total reads aligned to all adenovirus genomes. The pairwise nucleotide identities of several adenovirus subtypes to the TMAdv genome according to Chen et al. are given in parentheses. The Simian adenovirus 3 had the most reads aligned of all adenoviral genomes, which is consistent with its sequence similarity to the TMAdv. Additionally, the Human adenovirus D aligned the most reads of all human adenoviruses, which is consistent with the analysis of Chen et al. B) Inclusion of the Titi Monkey Adenovirus (TMAdv) in the target library resulted in the assignment of 12,568 reads to the TMAdv reference genome.