Skip to main content


Figure 2 | BMC Bioinformatics

Figure 2

From: Impact of variance components on reliability of absolute quantification using digital PCR

Figure 2

Example of the endpoint fluorescence for two dyes. In the left panel, the endpoint fluorescence of an artificial experiment without rain is shown, in the right panel the result of an artificial experiment with about 6% rain. For both dyes, the distribution is a mixture of two components, composed of output from both positive and negative partitions as shown with appropriate density functions on top and on the right of both graphs. An arbitrary threshold to separate both groups is added for each dye, dividing the area in four classification quadrants.

Back to article page