Figure 5
From: Impact of variance components on reliability of absolute quantification using digital PCR
Coverage of 95% confidence intervals of target concentration in the presence of pipette error. For a given concentration λ, the coverage was calculated as the ratio of the number of confidence intervals out of 1000 simulations that contain the true concentration λ divided by the total number of confidence intervals calculated (1000). The left panel shows results for confidence intervals calculated using a single sample method after pooling the partitions of the 8 technical replicates before estimating the concentration and its variance with Poisson statistics. The right panel shows results for confidence intervals calculated using a replicate based method. The concentration and its variance were calculated using the empirical mean and variance of the concentration estimates of 8 independent replicates. The pooled method shows a dramatic loss of coverage while the replicate based method shows correct coverage.